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In general, most of the cytokines detected in BALF were considerably increased in WT mice uncovered to PG with nicotine compared to PG alone or air management [42]. Some of these results have been discovered to be via nicotine activation of NF-κB signalling albeit in females but not in males. In addition, PG with nicotine triggered elevated macrophage and CD4+/CD8+ T-lymphocytes cell counts in BALF compared to air management, but these effects were ameliorated when animals were sub-chronically exposed to PG alone [42].
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